微量測試體系體積較小，持續測量過程中溶劑揮發會造成溶質濃度上升，引起表面張力漂移，要求作者說明揮發帶來的數據誤差如何控制，論證實驗結果可靠性。
二、完整論證邏輯
1.本研究采用Kibron氣泡壓力法進行檢測，該方法采集瞬時動態界面信號，不需要長時間靜態液面平衡，選取短時間內形成的穩態平臺數據開展分析，減少長時間測量帶來的揮發累積效應。
2.Kibron微量樣品槽為半密閉結構，液面上方能夠形成飽和水汽氛圍，有效降低溶劑揮發速率，緩解微體積體系溶質富集問題。
3.實驗統一固定平衡時長與數據截取區間，所有實驗組、空白對照組保持完全相同測試條件，若存在微弱揮發效應，屬于全域統一系統誤差，不會改變各組之間相對差異規律。
4.測試數據篩選標準統一，僅使用張力穩定平臺區間數值，剔除測試前后受波動影響的數據，進一步降低揮發趨勢帶來的干擾；平行樣品重復性良好，側面證明濃度未發生明顯偏移。
三、英文版審稿回復（可直接復制）
We appreciate the reviewer’s concern about solvent volatilization in micro-volume measurements. Multiple quality control measures were implemented to minimize such interference.
The Kibron bubble pressure method captures transient interfacial signals, and only stable plateau data obtained within a limited equilibrium period were adopted, avoiding continuous evaporation induced by prolonged static measurement. The micro sample chamber adopts a semi-closed design, which generates saturated vapor atmosphere above the liquid surface and suppresses solvent evaporation and concentration drift. All samples and blank controls were measured under identical parameters. Slight volatilization would lead to uniform systematic error and cannot alter the relative differences among experimental groups. Moreover, consistent data selection criteria were applied, and only steady-state signals were used for analysis. Therefore, the influence of volatilization can be regarded as negligible, and the surface tension data are comparable. Relevant control details have been supplemented in the revised manuscript.
四、中文版論文補充段落/回復文字
針對微量樣品測試過程中的溶劑揮發潛在干擾，本研究設置多重質控手段降低誤差。實驗使用Kibron動態氣泡壓力法檢測界面張力，依托瞬時信號采集選取短時間穩態平臺數據，避免長期靜置引發的溶質富集。微量樣品槽半密閉結構可以營造飽和水汽環境，抑制溶劑揮發。所有實驗組與空白對照組測試參數保持一致，微弱揮發帶來的影響屬于統一系統誤差，不會干擾組間差異性比較。同時本文統一截取穩態區間數據用于統計分析，平行樣品重復性良好，證實揮發造成的數據漂移可忽略。相關質控細節已在修訂稿方法部分補充。
五、實操避坑要點
不可表述為完全不存在揮發，采用“揮發干擾可忽略”的嚴謹說法；區分吊環靜態法與氣泡壓力法的差異，突出本儀器測試方式的優勢；不要單獨依靠單一論據，結合儀器結構、數據篩選、對照實驗形成完整證據鏈。
